Video of ICSI procedure for in vitro fertilization - IVF Treat male factor infertility by fertilizing eggs in the IVF lab
Advanced Fertility Center of Chicago IVF with ICSI specialist clinic
What is ICSI - intracytoplasmic sperm injection?
Intracytoplasmic means "into the cytoplasm" - the part of a cell that is inside the cell membrane.
- In an egg there is an outer shell, called the zona pellucida, and under that the oolemma or cell membrane (see picture below).
- Sperm are immobilized and picked up using a very small and sharp needle - and then injected into the cytoplasm of the egg.
- In many IVF programs intracytoplasmic sperm injection results in fertilization of about 70% to 80% of the eggs injected with sperm.
Intracytoplasmic sperm injection sperm injection technique - video
Steps shown in video (also see image below):
- Sperm is advanced to the tip of the needle
- Egg is penetrated and needle advanced
- Suction applied to break egg's outer membrane and mix sperm with egg's cytoplasm
- Sperm and egg cytoplasm mixture injected into egg
- Needle removed from egg
- Egg released from holding pipette
Explanation for video above Holding pipette at left (H) ICSI needle at right Sperm head is seen in the needle Cumulus cells on outer shell (zona) came from the ovarian follicle Polar body of egg is at 12 o'clock (polar body shows egg is "mature")
- 16 to 18 hours after the sperm are injected we check the eggs for evidence of fertilization
- Embryo transfer is done on day 3, or blastocyst transfer done day 5 after fertilization
- We do a pregnancy test 14 days after the day of intracytoplasmic sperm injection
- ICSI, when properly done, is a very effective procedure for obtaining fertilization with sperm from men with very low sperm counts, or low motility or very low normal morphology scores